High Resolution, High Throughput Electron Tomography Reconstruction
نویسندگان
چکیده
منابع مشابه
Semiautomatic, high-throughput, high-resolution protocol for three-dimensional reconstruction of single particles in electron microscopy.
In this chapter we describe the steps needed for reconstructing the three-dimensional structure of a macromolecular complex starting from its projections collected in electron micrographs. The concepts are shown through the use of Xmipp 3.0, a software suite specifically designed for the image processing of biological structures imaged with electron or X-ray microscopy. We illustrate the image ...
متن کاملHigh-resolution electron microscopy and electron tomography: resolution versus precision.
The performance of high-resolution electron microscopy and electron tomography is usually discussed in terms of two-point resolution, expressing the possibility of perceiving separately two image points of an object. However, the concept resolution obtains another meaning if one uses prior knowledge about the object and the imaging procedure in the form of a parametric model describing the expe...
متن کاملToward fully automated high-resolution electron tomography.
Electron tomography is a powerful tool in elucidating the three-dimensional architecture of large biological complexes and subcellular organelles. Its use can be expanded through the simplification of the tomographic procedure by automation of its tasks. In this paper, we describe our EMACT/EMCAT system, which automates both tomographic data collection and reconstruction.
متن کاملReconstruction of High-Resolution Computed Tomography Image in Sinogram Space
An important part of any computed tomography (CT) system is the reconstruction method, which transforms the measured data into images. Reconstruction methods for CT can be either analytical or iterative. The analytical methods can be exact, by exact projector inversion, or non-exact based on Back projection (BP). The BP methods are attractive because of thier simplicity and low computational co...
متن کاملHigh resolution image reconstruction in ultrasound computer tomography using deconvolution
Ultrasound computer tomography is an imaging method capable of producing volume images with high spatial resolution. The imaged object is enclosed by a cylindrical array of transducers. While one transducer emits a spherical wavefront (pulse), all other transducers are recording the radiofrequency (RF) a–scans simultaneously. Then another transducer acts as the emitter and so on. In this paper ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2005
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927605506330